TESTING OF HIGHLIGHTERS FLUORSCENCE
Lab: 1
Experiment: 1
Objective:
To accurately and precisely analyse the fluorescence of 3 given highlighters and check if they are fluorescent or not fluorescent using Fluorometer.
Final Results:
Table 1: Final results of fluorescence determination of Highlighters using Fluorometer.
SAMPLE EXCITATION WAVELENGH USED READING EMISSION WAVELENGTH READING
PINK HIGHLIGHTER 200nm-800nm 534.02 ALL WAVELENGTH 631.03
GREEN HIGHLIGHTER 200nm-800nm 204,245.93,402 ALL WAVELENGTH 511.94
BLUE HIGHLIGHTER 200nm-800nm 0 ALL WAVELENGTH 471.44
All of the highlighters were found to be fluorescent.
Discussion:
Deionised water was used as standard to check whether the coloured highlighters are fluorescent or not.
The analyses performed on highlighters are indeed fluorescent. The results obtained on comparing with the standard emissions are accurate and precise.
Thus with the results being positive, litigation against manufacturer must not be done.
References:
Durham College. Spectroscopy Manual (2017-2018), Oshawa.
FLUORESCENCE TESTING OF RIBOFLAVIN TABLETS
LAB: 1
EXPERIMENT: 2
Objective:
To accurately and precisely determine the amount of Riboflavin present in the B12 supplements via Fluorometer and compare it to claimed label.
Final Results:
Final results of quantitative measurement of Riboflavin in Jamieon tablets using Fluorometer and the percent label claim are shown in the table below.
Weights Percent label claim
Label Claim 100mg 100%
DC analysis tab 1 115mg 115%
DC analysis tab 2 117.5mg 117.5%
Discussion:
Various concentrations of Standard Riboflavin were prepared ranging from 1ppm to 100ppm to determine the concentration of riboflavin in tablet.
The analysis of tablet shows that it contains approximately same amount of the label claimed Riboflavin. Given the use of high correlation (R2 of 0.987) and standard curve of the standard prepared, the results obtained are quite accurate. Chloride and Bromide are chiefly responsible for quenching of fluorescence and may be responsible for decreased quantification of Riboflavin in tablets. Analysis of Chloride/halogen content must be done for precise and accurate results.
References:
Durham College: Spectroscopy Manual(2017-2018), Oshawa.
